(Proteasome Subunit Alpha Type 3 (PSMA3)) pH value is at the discretion of the manufacturer. We will discuss all possible options with you in detail to assure that you receive your protein of interest.ġ00 mM NaCL, 20 mM Hepes, 10% glycerol. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. As the protein has not been tested for functional studies yet we cannot offer a guarantee though. In addition to the applications listed above we expect the protein to work for functional studies as well. Please contact us if you require endotoxin removal. >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.Įndotoxin has not been removed. Eluate fractions are analyzed by SDS-PAGE and Western blot. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography.Eluate fractions are analyzed by SDS-PAGE. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant.Two step purification of proteins expressed in bacterial culture: We use the Expasy's protparam tool to determine the absorption coefficient of each protein. The concentration of the protein is calculated using its specific absorption coefficient. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of our recombinant proteins is measured using the absorbance at 280nm. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered. When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. fees might apply for the expression plasmid, the first expression experiments or purification optimization). In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. Our experts in the lab will ensure that you receive a correctly folded protein. This protein is a made to order protein and will be made for the first time for your order. State-of-the-art algorithm used for plasmid design (Gene synthesis).Coli) purified by multi-step, protein-specific process to ensure crystallization grade. Made in Germany - from design to production - by highly experienced protein experts. If you have a special request, please contact us. Tag location is at the discretion of the manufacturer. SSIGTGYDLS ASTFSPDGRV FQVEYAMKAV ENSSTAIGIR CKDGVVFGVE KLVLSKLYEE GSNKRLFNVD RHVGMAVAGL LADARSLADI AREEASNFRS NFGYNIPLKH LADRVAMYVH AYTLYSAVRP FGCSFMLGSY SVNDGAQLYM IDPSGVSYGY WGCAIGKARQ AAKTEIEKLQ MKEMTCRDIV KEVAKIIYIV HDEVKDKAFE LELSWVGELT NGRHEIVPKD IREEAEKYAK ESLKEEDESD DDNM This PSMA3 protein is labelled with His tag.ĮLISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys) Custom Recombinant Antibody (rAbs) Services.Annexin V-FITC Apoptosis Detection Kits.
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